Gag-Vpu cross talk modulating HIV-1 envelope incorporation and infectivity in cell-type dependent manner
نویسندگان
چکیده
Methods We introduced Vpu start codon mutation and point substitution in p17 Gag matrix (L30E) in HIV-1 pNLAD8 through PCR. Progeny viruses were produced in two cell-types: 293T and HeLa by transfection. Infectivity potential of Vpu+/Vpuviruses carrying Gag (L30E) mutation was assessed in TZM-bl cell line and their replication potential in peripheral blood mononuclear cells (PBMC) and monocyte-derived macrophages. The effect of mutation on virus release, envelope incorporation and infectivity was determined by RT ELISA and Western blot.
منابع مشابه
Evidence that Vpu Modulates HIV-1 Gag-Envelope Interaction towards Envelope Incorporation and Infectivity in a Cell Type Dependent Manner
The HIV-1 Vpu is required for efficient virus particle release from the plasma membrane and intracellular CD4 degradation in infected cells. In the present study, we found that the loss of virus infectivity as a result of envelope (Env) incorporation defect caused by a Gag matrix (MA) mutation (L30E) was significantly alleviated by introducing a start codon mutation in vpu. Inactivation of Vpu ...
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